The Truelab Quattro Real-Time Quantitative PCR Machine is designed for accurate and efficient nucleic acid quantification. It is typically used in research, clinical diagnostics, and quality control laboratories for applications requiring precise measurement of DNA or RNA.
1. High-Performance Detection:
- Real-Time Quantitative PCR: Offers real-time monitoring of PCR amplification, providing precise quantification of nucleic acids.
- Multi-Color Detection: Allows for simultaneous detection of multiple targets in a single run using different fluorescent dyes or probes.
2. Sample Throughput:
- Flexible Format: Usually supports various plate formats, such as 96-well plates, for high-throughput analysis.
3. User-Friendly Interface:
- Touchscreen or Computer-Based Interface: Provides an intuitive interface for setting up experiments, monitoring runs, and analyzing data.
4. Advanced Optics:
- Optical System: Utilizes advanced optics for accurate fluorescence detection and measurement of amplification signals.
5. Software Integration:
- Data Analysis Software: Comes with specialized software for real-time data analysis, including quantification, melt curve analysis, and statistical evaluations.
1. Sample Preparation:
- Prepare PCR Reactions: Mix DNA/RNA samples with primers, probes, and PCR reagents according to your experimental protocol.
2. Loading Samples:
- Insert Plates: Load the prepared samples into the PCR plate or other compatible formats.
3. Configure and Run PCR:
- Setup Protocol: Use the machine’s interface to set up the PCR protocol, including temperature cycles, fluorescence detection settings, and other parameters.
- Start the Run: Begin the PCR process. The machine will execute the program and collect data in real time.
4. Data Analysis:
- Analyze Results: Post-run, use the data analysis software to interpret results. This includes quantification of nucleic acids, analyzing amplification curves, and reviewing melt curves if applicable.